Article
Rapid detection of functional expression of C-5-DNA methyltransferases in yeast.
Nucleic acids research - 1 Mar 1998
Kladde M P, Simpson R T
Abstract excerpt
We have previously employed the cytosine-5-DNA methyltransferase (MTase), M. Sss I, as a probe for chromatin architecture in intact cells. Although M. Sss I offers the highest resolution of any currently available MTase, the difficulty in establishing stable, methylation-positive strains poses a barrier to its general utility as a chromatin probe. We describe a simple screen for M. Sss I-expressing strains that...
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