Article

Chemoenzymatic labeling of DNA methylation patterns for single-molecule epigenetic mapping

2021-02-24

Abstract excerpt

<h4>ABSTRACT</h4> DNA methylation, specifically, methylation of cytosine (C) nucleotides at the 5-carbon position (5-mC), is the most studied and among the most significant epigenetic modifications. Here we developed a chemoenzymatic procedure to fluorescently label non-methylated cytosines in the CpG context allowing epigenetic profiling of single DNA molecules spanning hundreds of thousands of base pairs. For t...

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Literature Corpus work: c14af428-1b16-5017-a0e0-e21f2e34001f
DOI: 10.1101/2021.02.24.432628

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