Article
Validation of HLA-DR locus typing in forensic specimens by combining PCR-SSP with PCR-RFLP.
Journal of forensic sciences - 1 Sept 1997
Ota M, Katsuyama Y, Liu C Y, Arakura A, Fukushima H
Abstract excerpt
The applicability of HLA-DR DNA typing combined with PCR-SSP (sequence specific primers) and PCR-RFLP (restriction fragment length polymorphism) to forensic practice was investigated. PCR-SSP was as effective as serological HLA-DR typing in determining DR types. For more precise definition of DRB...
Topics
- Base Sequence
- Blood Stains
- DNA
- DNA Fingerprinting
- Gene Amplification
- Gene Frequency
- Genotype
- HLA-DR Antigens
- Hair
- Humans
- Japan
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length
- Sensitivity and Specificity
- Tooth