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Article

An improved strategy for HLA-DRB1 subtyping by digestion of PCR-amplified DNA with allele-specific restriction endonucleases.

Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society - 1 Jan 1995

Danzé P M, Bianchi F, Fajardy I, Rousseaux J

Abstract excerpt

We developed a simple, rapid and inexpensive method of DRB1 alleles genotyping by digestion of amplified DNA with allele-specific restriction fragments endonucleases. We took advantage of this protocol, initially described by Yunis et al. (1991) and called AFLP (Amplification Length Fragment Polymorphism) to standardise amplification procedure. Typing strategy was particularly studied to limit the number of...

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PMID: 8534755

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