Article
DNA typing system for HLA-A2 alleles by polymerase chain reaction with sequence-specific primers.
Chinese medical sciences journal = Chung-kuo i hsueh k'o hsueh tsa chih - 1 Sept 2001
Zhang Q, Zhai N, Geng L, Song F
Abstract excerpt
OBJECTIVE: To establish a PCR-SSP method for discriminating as many HLA-A*02 alleles, which could easily be introduced into a routine laboratory. METHODS: In this study we typed HLA-A*02 polymorphisms by a sequence-specific primer (SSP) method, which involved round 1 and round 2 PCR reactions to detect 17 HLA-A*02 alleles (they are HLA-A*0201-0217 alleles) covering exon 2 and exon 3. RESULTS: We have found that...
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