Article
Determination of DQB1 alleles using PCR amplification and allele-specific primers.
European journal of immunogenetics : official journal of the British Society for Histocompatibility and Immunogenetics - 1 Oct 1995
Lepage V, Ivanova R, Loste M N, Mallet C, Douay C, Naoumova E, Charron D
Abstract excerpt
Molecular genotyping of HLA class II genes is commonly carried out using polymerase chain reaction (PCR) in combination with sequence-specific oligotyping (PCR-SSO) or a combination of the PCR and restriction fragment length polymorphism methods (PCR-RFLP). However, the identification of the DQB1...
Topics
- Alleles
- Base Sequence
- Cell Line
- DNA Primers
- HLA-DQ Antigens
- HLA-DQ beta-Chains
- Humans
- Lymphocytes
- Molecular Sequence Data
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length