Article
Determination of DQB1 alleles using PCR amplification and allele-specific primers.
European journal of immunogenetics : official journal of the British Society for Histocompatibility and Immunogenetics - 1 Oct 1995
Lepage V, Ivanova R, Loste M N, Mallet C, Douay C, Naoumova E, Charron D
Abstract excerpt
Molecular genotyping of HLA class II genes is commonly carried out using polymerase chain reaction (PCR) in combination with sequence-specific oligotyping (PCR-SSO) or a combination of the PCR and restriction fragment length polymorphism methods (PCR-RFLP). However, the identification of the DQB1 type by PCR-SSO and PCR-RFLP is very time-consuming which is disadvantageous for the typing of cadaveric organ donors....
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