Article
[Simple and rapid detection of HLA-DRB polymorphism from forensic samples].
Nihon hoigaku zasshi = The Japanese journal of legal medicine - 1 Aug 1998
Kimura T, Nata M, Hashiyada M, Funayama M, Sagisaka K
Abstract excerpt
Simple and rapid detection of HLA-DRB polymorphism has been performed using AMPLICOR HLA-DRB Typing Kit. We tried to apply this kit to various forensic samples. When DNA was extracted from the forensic samples using conventional phenol-chloroform method, addition of 7.5 mM MgCl2 was required to PCR amplification. HLA-DRB types were detected from DNA more than 0.1 ng by PCR amplification. Typing of unrelated 50...
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