Article
Rapid HLA-DR genotyping by PCR-amplification with sequence-specific primers.
Chinese medical journal - 1 Sept 1996
Tan J, Xie T, Xu Q, Xu D, Wang X
Abstract excerpt
OBJECTIVE: To establish a rapid genotyping for HLA-DR alleles by polymerase chain reaction with sequence-specific primers (PCR-SSP) for clinical application. MATERIAL AND METHODS: The subjects of study included 69 recipients, 43 unrelated donors and 5 cell lines. Genomic DNA was prepared from peripheral blood leukocytes by a salting-out method. Thirty primers were designed according to the HLA-DRB nucleotide...
Topics
- Alleles
- Genotype
- Graft Survival
- HLA-DR Antigens
- Humans
- Kidney Transplantation
- Polymerase Chain Reaction
- Preoperative Care
- Sensitivity and Specificity