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Article

Rapid HLA-DR genotyping by PCR-amplification with sequence-specific primers.

Chinese medical journal - 1 Sept 1996

Tan J, Xie T, Xu Q, Xu D, Wang X

Abstract excerpt

OBJECTIVE: To establish a rapid genotyping for HLA-DR alleles by polymerase chain reaction with sequence-specific primers (PCR-SSP) for clinical application. MATERIAL AND METHODS: The subjects of study included 69 recipients, 43 unrelated donors and 5 cell lines. Genomic DNA was prepared from peripheral blood leukocytes by a salting-out method. Thirty primers were designed according to the HLA-DRB nucleotide...

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PMID: 9275342

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