Article
A nested PCR-RFLP method for high-resolution typing of HLA-A alleles.
European journal of immunogenetics : official journal of the British Society for Histocompatibility and Immunogenetics - 1 Feb 1998
Mitsunaga S, Tokunaga K, Kashiwase K, Akaza T, Tadokoro K, Juji T
Abstract excerpt
We developed a nested polymerase chain reaction (PCR) restriction fragment length polymorphism (RFLP) method for high-resolution typing of HLA-A alleles. HLA-A alleles can be identified by this method without the need for other information such as serological type. The first PCR was performed usi...
Topics
- Alleles
- DNA
- DNA Restriction Enzymes
- Electrophoresis, Polyacrylamide Gel
- Exons
- Genes, MHC Class I
- HLA-A Antigens
- Heterozygote
- Histocompatibility Testing
- Homozygote
- Humans
- Japan
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length
- Polymorphism, Single-Stranded Conformational