Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

A nested PCR-RFLP method for high-resolution typing of HLA-A alleles.

European journal of immunogenetics : official journal of the British Society for Histocompatibility and Immunogenetics - 1 Feb 1998

Mitsunaga S, Tokunaga K, Kashiwase K, Akaza T, Tadokoro K, Juji T

Abstract excerpt

We developed a nested polymerase chain reaction (PCR) restriction fragment length polymorphism (RFLP) method for high-resolution typing of HLA-A alleles. HLA-A alleles can be identified by this method without the need for other information such as serological type. The first PCR was performed using outer primers, ASP5 and ASP3, specific for the HLA-A gene, and a 991-bp DNA fragment extending from exon 1 through...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Alleles
Share to DNA

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Electrophoresis, Polyacrylamide Gel

Share to Heterozygote

Share to Homozygote

Share to Polymerase Chain Reaction

Share to Polymorphism, Restriction Fragment Length

Source

Identifiers

PMID: 9587741
DOI: 10.1046/j.1365-2370.1998.00093.x

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

A nested PCR-RFLP method for high-resolution typing of HLA-A alleles.PMID 9587741Read ↗

Typing class I HLA-A gene using a nested PCR-RFLP procedure.Semantic proximity Read ↗

An improved strategy for HLA-DRB1 subtyping by digestion of PCR-amplified DNA with allele-specific restriction endonucleases.Semantic proximity Read ↗

Complete HLA-A DNA typing using the PCR-RFLP method combined with allele group- and sequence-specific amplification.Semantic proximity Read ↗

A comprehensive PCR-SSP typing system for identification of HLA-A locus alleles.Semantic proximity Read ↗

Validation of HLA-DR locus typing in forensic specimens by combining PCR-SSP with PCR-RFLP.Semantic proximity Read ↗

Allele typing of HLA-A10 group by nested-PCR-low ionic strength single stranded conformation polymorphism and a novel A26 allele (A26KY, A*2605).Semantic proximity Read ↗

A PCR-SSP method to specifically select HLA-A*0201 individuals for immunotherapeutic studies.Semantic proximity Read ↗

DNA typing of the HLA-A gene: population study and identification of four new alleles in Japanese.Semantic proximity Read ↗

DNA typing system for HLA-A2 alleles by polymerase chain reaction with sequence-specific primers.Semantic proximity Read ↗

A simple and rapid method for HLA-DRB and -DQB typing by digestion of PCR-amplified DNA with allele specific restriction endonucleases.Semantic proximity Read ↗

Multiplex ARMS-PCR-RFLP method for high-resolution typing of HLA-DRB1.Semantic proximity Read ↗

Cost-efficient multiplex PCR for routine genotyping of up to nine classical HLA loci in a single analytical run of multiple samples by next generation sequencing.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.