Article
Determination of DRB alleles using PCR amplification and allele-specific primers.
European journal of immunogenetics : official journal of the British Society for Histocompatibility and Immunogenetics - 1 Feb 1994
Lepage V, Schaeffer V, Mallet C, Ivanova R, Khalil I, Charron D
Abstract excerpt
HLA class-II allelic diversity is commonly defined using polymerase chain reaction (PCR) in combination with sequence-specific oligotyping (PCR-SSO) or the combination of PCR and restriction fragment length polymorphism methods (PCR-RFLP). Nevertheless, the identification of the DRB polymorphism...
Topics
- Alleles
- Cell Line
- DNA Primers
- HLA-DR Antigens
- Humans
- Polymerase Chain Reaction
- Recombination, Genetic
- Reproducibility of Results
- Sensitivity and Specificity