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HLA-DR typing by polymerase chain reaction amplification with sequence-specific primers in paternity disputes.

Journal of the Chinese Medical Association : JCMA - 1 Feb 2003

Lin Jeong-Shi, Tzeng Cheng-Hwai, Huang De-Feng, Lyou Jau-Yi, Chen Ying-Ju, Hu Hui-Yu, Yung Chien-Hui

Abstract excerpt

BACKGROUND: HLA-DR typing was generally performed by serology before. HLA-DRB1 typing can be achieved by polymerase chain reaction amplification with sequence specific primers (PCR-SSP). METHODS: In this study, primers for "low-resolution" HLA-DR typing by PCR-SSP were synthesized upon our request by a company in Taiwan. Twenty-eight DNA samples from international standardized DNA Reference Panel and 20 DNA...

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PMID: 12716004

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