Article
Isolation of animal cell mutants defective in long-chain fatty aldehyde dehydrogenase. Sensitivity to fatty aldehydes and Schiff's base modification of phospholipids: implications for Sj-ogren-Larsson syndrome.
The Journal of biological chemistry - 19 Sept 1997
James P F, Zoeller R A
Abstract excerpt
Using tritium suicide, we have isolated a variant of the Chinese hamster ovary cell line, CHO-K1, that is deficient in long-chain fatty alcohol:NAD+ oxidoreductase (FAO; EC 1.1.1.192). Specifically, it was the fatty aldehyde dehydrogenase component that was affected. The enzymatic deficiency foun...
Topics
- Aldehyde Oxidoreductases
- Aldehydes
- Animals
- CHO Cells
- Cell Line
- Cricetinae
- Genetic Complementation Test
- Humans
- Mutation
- Phosphatidylethanolamines
- Schiff Bases
- Sjogren-Larsson Syndrome