Article
A fibroblast cell line defective in alkyl-dihydroxyacetone phosphate synthase: a novel defect in plasmalogen biosynthesis.
Proceedings of the National Academy of Sciences of the United States of America - 29 Apr 1997
Nagan N, Hajra A K, Das A K, Moser H W, Moser A, Lazarow P, Purdue P E, Zoeller R A
Abstract excerpt
Using fluorescence-activated cytotoxicity selection, followed by colony autoradiographic screening of the surviving population, we have isolated a unique plasmalogen-deficient Chinese hamster ovary (CHO) cell line. The mutant, NZel-1, showed a dramatic (90%) reduction in the rate of biosynthesis...
Topics
- Alkyl and Aryl Transferases
- Animals
- CHO Cells
- Cell Compartmentation
- Cricetinae
- Fibroblasts
- Genes, Recessive
- Genetic Complementation Test
- Microbodies
- Models, Biological
- Mutagenesis
- Mutation
- Plasmalogens
- Selection, Genetic
- Transferases