Article
A fibroblast cell line defective in alkyl-dihydroxyacetone phosphate synthase: a novel defect in plasmalogen biosynthesis.
Proceedings of the National Academy of Sciences of the United States of America - 29 Apr 1997
Nagan N, Hajra A K, Das A K, Moser H W, Moser A, Lazarow P, Purdue P E, Zoeller R A
Abstract excerpt
Using fluorescence-activated cytotoxicity selection, followed by colony autoradiographic screening of the surviving population, we have isolated a unique plasmalogen-deficient Chinese hamster ovary (CHO) cell line. The mutant, NZel-1, showed a dramatic (90%) reduction in the rate of biosynthesis and levels of plasmalogens, as determined using short- and long-term labeling with 32Pi. Enzymatic assays and lipid...
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