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Article

A 25-hydroxycholesterol-resistant cell line deficient in acyl-CoA: cholesterol acyltransferase.

The Journal of biological chemistry - 5 Jul 1991

Metherall J E, Ridgway N D, Dawson P A, Goldstein J L, Brown M S

Abstract excerpt

We describe a line of mutant Chinese hamster ovary cells, SRD-4 cells, that lacks acyl-coenzyme A:cholesterol acyltransferase (ACAT) activity and fails to synthesize cholesteryl esters when stimulated with 25-hydroxycholesterol or low density lipoprotein. The cells also have a partial defect in their ability to repress transcription of three sterol-regulated genes, 3-hydroxy-3-methylglutaryl-coenzyme A synthase,...

Topics

Amides
Animals
Cell Line
Cholesterol Esters
Cricetinae
Cricetulus
Homeostasis
Hydroxycholesterols
Hydroxymethylglutaryl-CoA Synthase
Mutation
Organosilicon Compounds

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

RNA

Receptors, LDL

Sterol O-Acyltransferase

Sterols

Source

Identifiers

PMID: 1712025

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1712025-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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