Article
Down-regulation of mitochondrial acyl carrier protein in mammalian cells compromises protein lipoylation and respiratory complex I and results in cell death.
The Journal of biological chemistry - 24 Apr 2009
Feng Dejiang, Witkowski Andrzej, Smith Stuart
Abstract excerpt
The objective of this study was to evaluate the physiological importance of the mitochondrial fatty acid synthesis pathway in mammalian cells using the RNA interference strategy. Transfection of HEK293T cells with small interfering RNAs targeting the acyl carrier protein (ACP) component reduced ACP mRNA and protein levels by >85% within 24 h. The earliest phenotypic changes observed were a marked decrease in the...
Topics
- Acyl Carrier Protein
- Catalysis
- Cell Death
- Cell Proliferation
- Cytoplasm
- Down-Regulation
- Electron Transport
- Electron Transport Complex I
- Humans
- Membrane Potentials
- Mitochondria
- Mutation
- Oxidative Stress
- Phenotype
- Thioctic Acid