Article
A rapid selection for animal cell mutants with defective peroxisomes.
Biochimica et biophysica acta - 16 May 1990
Morand O H, Allen L A, Zoeller R A, Raetz C R
Abstract excerpt
Chinese hamster ovary (CHO) cells take up and incorporate 9-(1'-pyrene)nonanol (P9OH) into phospholipids and neutral lipids. Exposure of P9OH-labeled cells to long wavelength ultraviolet (UV) light causes cell death, because excitation of the pyrene moiety generates reactive oxygen species. CHO mutant cells deficient in plasmalogen biosynthesis and peroxisome assembly (Zoeller, R.A. and Raetz, C.R.H. (1986) Proc....
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