Article
A functional assay in yeast for the human estrogen receptor displays wild-type and variant estrogen receptor messenger RNAs present in breast carcinoma.
Cancer research - 15 Aug 1997
van Dijk M A, Floore A N, Kloppenborg K I, van 't Veer L J
Abstract excerpt
Human estrogen receptor (hER) variants or mutants with altered functional activity may be responsible for resistance to the antiestrogen tamoxifen in breast cancer. The method presented in this report is a screening method for functional activity of hER in yeast Saccharomyces cerevisiae. hER mRNA...
Topics
- Biological Assay
- Breast Neoplasms
- DNA, Complementary
- DNA, Neoplasm
- Female
- Genetic Vectors
- Humans
- Immunohistochemistry
- Neoplasm Proteins
- Phenotype
- Polymerase Chain Reaction
- RNA, Messenger
- Receptors, Estrogen
- Receptors, Progesterone
- Saccharomyces cerevisiae
- Tumor Cells, Cultured