Article
A highly conserved region in the hormone-binding domain of the human estrogen receptor functions as an efficient transactivation domain in yeast.
Gene - 10 Jun 1994
Pierrat B, Heery D M, Chambon P, Losson R
Abstract excerpt
Human estrogen receptor (hER) mutants which activate transcription in the absence of hormone were isolated by random mutagenesis and genetic selection in the yeast Saccharomyces cerevisiae. Twenty constitutive hER mutants defining ten different alleles were selected. All sequence changes resulted...
Topics
- Amino Acid Sequence
- Binding Sites
- Conserved Sequence
- Genes, Reporter
- Humans
- Molecular Sequence Data
- Mutation
- Phenotype
- Receptors, Estrogen
- Saccharomyces cerevisiae
- Sequence Homology, Amino Acid
- Transcriptional Activation