Functional analysis of the human estrogen receptor using a phenotypic transactivation assay in yeast.

doi:10.1016/0378-1119(92)90277-v

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Article

Functional analysis of the human estrogen receptor using a phenotypic transactivation assay in yeast.

Gene - 1 Oct 1992

Pierrat B, Heery D M, Lemoine Y, Losson R

Abstract excerpt

We have constructed yeast strains in which the expression of the Saccharomyces cerevisiae URA3 gene is induced by the human estrogen receptor (hER). Promoter sequences required for both basal and activated transcription of URA3 were replaced with one or three estrogen-response elements (EREs) pos...

Topics

Blotting, Western
Cloning, Molecular
Estradiol
Humans
Orotidine-5'-Phosphate Decarboxylase
Phenotype
Receptors, Estrogen
Saccharomyces cerevisiae
Trans-Activators
Transcriptional Activation

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