Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Functional analysis of the human estrogen receptor using a phenotypic transactivation assay in yeast.

Gene - 1 Oct 1992

Pierrat B, Heery D M, Lemoine Y, Losson R

Abstract excerpt

We have constructed yeast strains in which the expression of the Saccharomyces cerevisiae URA3 gene is induced by the human estrogen receptor (hER). Promoter sequences required for both basal and activated transcription of URA3 were replaced with one or three estrogen-response elements (EREs) positioned upstream of the native TATA box. These constructs were each integrated at the TRP1 locus of a yeast strain in...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Blotting, Western
Share to Cloning, Molecular
Share to Estradiol

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Phenotype

Share to Receptors, Estrogen

Share to Saccharomyces cerevisiae

Share to Trans-Activators

Share to Transcriptional Activation

Source

Identifiers

PMID: 1398105
DOI: 10.1016/0378-1119(92)90277-v

Genes

TAF2
TAF1

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Functional analysis of the human estrogen receptor using a phenotypic transactivation assay in yeast.PMID 1398105Read ↗

A highly conserved region in the hormone-binding domain of the human estrogen receptor functions as an efficient transactivation domain in yeast.Semantic proximity Read ↗

A highly efficient and sensitive screening method for trans-activation activity of estrogen receptors.Semantic proximity Read ↗

Probing the structure and function of the estrogen receptor ligand binding domain by analysis of mutants with altered transactivation characteristics.Semantic proximity Read ↗

Helix 12 in the human estrogen receptor (hER) is essential for the hER function by overcoming nucleosome repression in yeast.Semantic proximity Read ↗

Role for Hsp90-associated cochaperone p23 in estrogen receptor signal transduction.Semantic proximity Read ↗

High level expression of biologically active estrogen receptor in Saccharomyces cerevisiae.Semantic proximity Read ↗

The analysis of chimeric human/rainbow trout estrogen receptors reveals amino acid residues outside of P- and D-boxes important for the transactivation function.Semantic proximity Read ↗

Identification of a third autonomous activation domain within the human estrogen receptor.Semantic proximity Read ↗

Structure-function analysis of the hormone binding domain of the human estrogen receptor by region-specific mutagenesis and phenotypic screening in yeast.Semantic proximity Read ↗

A functional assay in yeast for the human estrogen receptor displays wild-type and variant estrogen receptor messenger RNAs present in breast carcinoma.Semantic proximity Read ↗

A genetic, non-transcriptional assay for nuclear receptor ligand binding in yeast.Semantic proximity Read ↗

Estrogen receptor variants ERdelta5 and ERdelta7 down-regulate wild-type estrogen receptor activity.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.