Screening patients for heterozygous p53 mutations using a functional assay in yeast.

doi:10.1038/ng1093-124

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Article

Screening patients for heterozygous p53 mutations using a functional assay in yeast.

Nature genetics - 1 Oct 1993

Ishioka C, Frebourg T, Yan Y X, Vidal M, Friend S H, Schmidt S, Iggo R

Abstract excerpt

Inherited mutations of the p53 gene significantly increase the risk of developing diverse malignancies, and germline p53 mutations can be detected by assaying the transcriptional activity of the p53 protein in mammalian cells. Here we describe a method starting with lymphocytes that allows detect...

Topics

Animals
Base Sequence
DNA
Genetic Testing
Genetic Vectors
Heterozygote
Lymphocytes
Molecular Sequence Data
Mutation
Polymerase Chain Reaction
Saccharomyces cerevisiae
Templates, Genetic
Transcription, Genetic
Tumor Suppressor Protein p53

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