Article
Screening patients for heterozygous p53 mutations using a functional assay in yeast.
Nature genetics - 1 Oct 1993
Ishioka C, Frebourg T, Yan Y X, Vidal M, Friend S H, Schmidt S, Iggo R
Abstract excerpt
Inherited mutations of the p53 gene significantly increase the risk of developing diverse malignancies, and germline p53 mutations can be detected by assaying the transcriptional activity of the p53 protein in mammalian cells. Here we describe a method starting with lymphocytes that allows detection of germline p53 mutations by 'functional' analysis of p53 protein expressed in Saccharomyces cerevisiae. The p53...
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