Article
High level expression of biologically active estrogen receptor in Saccharomyces cerevisiae.
The Journal of steroid biochemistry and molecular biology - 1 Sept 1991
McDonnell D P, Nawaz Z, Densmore C, Weigel N L, Pham T A, Clark J H, O'Malley B W
Abstract excerpt
Biochemical over-expression of the human estrogen receptor was achieved using a Saccharomyces cerevisiae expression system. The receptor was produced as a novel ubiquitin fusion protein. This fusion protein is short lived in the cell and is processed to produce unfused receptor shortly after folding. Conventional high copy expression plasmids produced receptor to about 0.04% of the total soluble protein. By...
Topics
- Alleles
- Animals
- Blotting, Western
- CHO Cells
- Cloning, Molecular
- Cricetinae
- Cricetulus
- Estrogens
- Gene Expression
- Genes, Fungal
- Humans