Article
Generation of novel human MHC class II mutant B-cell lines by integrating YAC DNA into a cell line homozygously deleted for the MHC class II region.
Human molecular genetics - 1 Aug 1997
Fabb S A, Davies A F, Correa I, Kelly A P, Mackie C, Trowsdale J, Ragoussis J
Abstract excerpt
The human B lymphoblastoid cell line (LCL) 721.174 sustains a large homozygous deletion in the major histocompatibility complex (MHC) class II region that results in an absence of DQ and DR molecules as well as a deficiency in the assembly and transport of class I molecules to the cell surface. T...
Topics
- Amino Acid Sequence
- B-Lymphocytes
- Cell Line
- Chromosomes, Artificial, Yeast
- DNA
- Gene Dosage
- Gene Expression
- Genetic Variation
- Histocompatibility Antigens Class II
- Homozygote
- Humans
- Karyotyping
- Major Histocompatibility Complex
- Molecular Sequence Data
- Mutagenesis, Insertional
- Sequence Deletion
- Transfection