MHC class II deletion mutant expresses normal levels of transgene encoded class II molecules that have abnormal conformation and impaired antigen presentation ability.

Abstract excerpt

Successive transfers of HLA-DR alpha and beta genes restored expression of HLA-DR antigens to human B-lymphoblastoid cell line, LCL .174, from which all known expressible class II genes are deleted. While transferent cells displayed large amounts of DR on their surfaces, transgene-encoded DR3 molecules lacked a conformation-dependent epitope. DR1-restricted CTL lysis of DR1-expressing transferents pulsed with...

Topics

• Antibodies, Monoclonal
• Antigen-Presenting Cells
• Carrier Proteins
• Chromosome Deletion
• Genes, MHC Class II
• HLA-DR Antigens
• Humans
• In Vitro Techniques
• Major Histocompatibility Complex
• Mutation