Article
Isolation and characterization of IS1 circles.
Gene - 3 Jun 1997
Sekine Y, Eisaki N, Kobayashi K, Ohtsubo E
Abstract excerpt
Transposase encoded by insertion sequence IS1 is produced from two out-of-phase reading frames by translational frameshifting that occurs in a run of adenines. An IS1 mutant with a single adenine insertion in the run of adenines efficiently produces transposase, resulting in generation of miniplasmids by deletion for a region adjacent to IS1 from a plasmid carrying the IS1 mutant. Here, we found that besides...
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