Article
Evidence for a role of translational frameshifting in the expression of transposition activity of the bacterial insertion element IS1.
Gene - 30 Mar 1990
Lüthi K, Moser M, Ryser J, Weber H
Abstract excerpt
The bacterial insertion element IS1 contains two essential open reading frames, insA and insB, arranged in tandem. We have introduced a number of site-specific mutations into the region including the 3'-terminal region of insA, the region between insA and insB, and the ATG codon at the start of insB. Relative transposition activities of mutant and wild-type elements were determined using a modified in vivo...
Topics
- Amino Acid Sequence
- Base Sequence
- Chromosome Mapping
- DNA Transposable Elements
- Escherichia coli
- Gene Expression Regulation
- Molecular Sequence Data
- Mutation
- Plasmids
- Protein Biosynthesis
- Ribosomes