Article
Functional organization of the ends of IS1: specific binding site for an IS 1-encoded protein.
Molecular microbiology - 1 Sept 1990
Zerbib D, Prentki P, Gamas P, Freund E, Galas D J, Chandler M
Abstract excerpt
The IS 1-encoded protein InsA binds specifically to both ends of IS1, and acts as a repressor of IS1 gene expression and may be a direct inhibitor of the transposition process. We show here, using DNasel 'foot-printing' and gel retardation, that the InsA binding sites are located within the 24/25 bp minimal active ends of IS1 and that InsA induces DNA bending upon binding. Conformational modification of the ends...
Topics
- Bacterial Proteins
- Base Sequence
- Binding Sites
- DNA Transposable Elements
- DNA-Binding Proteins
- Deoxyribonuclease I
- Escherichia coli Proteins
- Integration Host Factors
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation