IS911-mediated transpositional recombination in vitro.

doi:10.1006/jmbi.1996.0624

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Article

IS911-mediated transpositional recombination in vitro.

Journal of molecular biology - 22 Nov 1996

Polard P, Ton-Hoang B, Haren L, Bétermier M, Walczak R, Chandler M

Abstract excerpt

A cell-free system is described that accomplishes an unusual type of transposition/recombination involving the bacterial insertion sequence IS911. Using a plasmid substrate carrying a derivative of IS911, we show that bacterial cell extracts enriched for the IS911 transposase, OrfAB, carry out a...

Topics

Bacterial Proteins
Base Sequence
DNA Nucleotidyltransferases
DNA Transposable Elements
DNA, Bacterial
Escherichia coli
Escherichia coli Proteins
Integrases
Models, Biological
Mutation
Nucleic Acid Conformation
Plasmids
Recombination, Genetic
Retroviridae
Shigella dysenteriae
Transposases

Source

Open in PubMed

Identifiers

PMID: 8950268
DOI: 10.1006/jmbi.1996.0624

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-8950268-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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