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Article

Characterization of insertion sequence IS946, an Iso-ISS1 element, isolated from the conjugative lactococcal plasmid pTR2030.

Journal of bacteriology - 1 Aug 1990

Romero D A, Klaenhammer T R

Abstract excerpt

The self-transmissible plasmid pTR2030 mobilized nonconjugative heterologous cloning vectors pGK12 (Cmr Emr) and pSA3 (Emr) at frequencies of 10(-5) to 10(-6) per input donor. Transconjugants harbored a 51- or 58-kilobase (kb) plasmid not found in the parental strains that cotransferred at high frequency with Cmr Emr and pTR2030-encoded phage resistance (Hsp+) in second-round matings (10(-1) per input donor)....

Topics

Amino Acid Sequence
Base Sequence
Cloning, Molecular
Conjugation, Genetic
DNA Transposable Elements
Escherichia coli
Lactococcus lactis
Molecular Sequence Data
Phenotype
Plasmids
R Factors

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Restriction Mapping

Sequence Homology, Nucleic Acid

Source

Open in PubMed Open full text

Identifiers

PMID: 2165471
DOI: 10.1128/jb.172.8.4151-4160.1990
PMCID: PMC213237

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2165471-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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