Article
IS911-mediated intramolecular transposition is naturally temperature sensitive.
Molecular microbiology - 1 Aug 1997
Haren L, Bétermier M, Polard P, Chandler M
Abstract excerpt
It is shown here that the bacterial insertion sequence IS911 exhibits a temperature-sensitive transposition phenotype. Previous results have demonstrated that elevated levels of the IS911 transposase OrfAB generate significant quantities of a figure-eight form, created by cleavage and circularization of one of the transposon strands, and of an excised circular form, in which both transposon strands have been...
Topics
- Bacterial Proteins
- Base Sequence
- Cloning, Molecular
- DNA Transposable Elements
- DNA, Bacterial
- DNA, Circular
- Escherichia coli
- Escherichia coli Proteins
- Genes, Bacterial
- Lac Operon
- Nucleic Acid Conformation