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Article

Site-specific transposition of insertion sequence IS630.

Journal of bacteriology - 1 Jul 1990

Tenzen T, Matsutani S, Ohtsubo E

Abstract excerpt

IS630 is a 1.15-kilobase sequence in Shigella sonnei that, unlike many mobile elements, seems not to mediate cointegration between different replicons. To assess its transposition, we constructed composite elements containing inverted copies of IS630 flanking a drug resistance gene. We found that these composite elements transposed to plasmid ColE1 in Escherichia coli. DNA sequencing showed that transposition...

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Identifiers

PMID: 2163390
DOI: 10.1128/jb.172.7.3830-3836.1990
PMCID: PMC213363

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