Article
In vitro characterization of the splicing efficiency and fidelity of the RmInt1 group II intron as a means of controlling the dispersion of its host mobile element.
RNA (New York, N.Y.) - 1 Dec 2014
Chillón Isabel, Molina-Sánchez María Dolores, Fedorova Olga, García-Rodríguez Fernando Manuel, Martínez-Abarca Francisco, Toro Nicolás
Abstract excerpt
Group II introns are catalytic RNAs that are excised from their precursors in a protein-dependent manner in vivo. Certain group II introns can also react in a protein-independent manner under nonphysiological conditions in vitro. The efficiency and fidelity of the splicing reaction is crucial, to guarantee the correct formation and expression of the protein-coding mRNA. RmInt1 is an efficient mobile intron found...
Topics
- DNA Transposable Elements
- Exons
- Introns
- Mutation
- Nucleic Acid Conformation
- RNA Precursors
- RNA Splicing
- RNA, Catalytic
- Sinorhizobium meliloti