Article
HLA-DRB and -DBQ typing by PCR amplification using sequence-specific primers (PCR-SSP): assessment after 1 year of routine use by three laboratories.
European journal of immunogenetics : official journal of the British Society for Histocompatibility and Immunogenetics - 1 Apr 1994
Mercier B, al Daccak R, Samaan A, David F, Carta A, Cracco P, Raguenes O, Dufosse F, Ferec C, Charron D
Abstract excerpt
Using sequence-specific amplifications, a practical and fast technique for DRB and DQB typing has been developed. The primers are chosen in order to amplify groups of alleles corresponding to the same serological specificity. In a second step, precise allelic determination is obtained by studying the restriction fragment length polymorphism of the PCR products. The experience of three laboratories using this...
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