Article
[Rapid genotyping for HLA-DR by PCR-amplification with sequence-specific primers and clinical practice].
Zhonghua wai ke za zhi [Chinese journal of surgery] - 1 Jan 1996
Tan J, Xie T, Xu Q
Abstract excerpt
Genotyping for HLA-DR alleles by polymerase chain reaction with sequence-specific primers (PCR-SSP) was first typed in 112 individuals of donor-recipients of cadaveric transplantation and 9 cell lines DNA. Twenty separate PCR reactions were performed per sample. The amplification was accomplished by 34 cycles consisting of denaturation at 94 degrees C for 30 sec, annealing at 60 degrees C for 50 sec and extension...
Topics
- Alleles
- Genotype
- HLA-DR Antigens
- Humans
- Kidney Transplantation
- Polymerase Chain Reaction