Article
Discrimination of human HLA-DRB1 alleles by PCR-SSCP (single-strand conformation polymorphism) method.
European journal of immunogenetics : official journal of the British Society for Histocompatibility and Immunogenetics - 1 Feb 1994
Bannai M, Tokunaga K, Lin L, Kuwata S, Mazda T, Amaki I, Fujisawa K, Juji T
Abstract excerpt
A single-strand conformation polymorphism (PCR-SSCP) method has been adopted for discrimination of human HLA-DRB1 alleles. This method enabled the detection of DNA polymorphisms including point mutations at a variety of positions in the DNA fragments of the HLA-DRB1 gene. A total of 27 HLA-DRB1 alleles from 172 healthy donors were analysed using a combination of PCR-SSCP with group-specific amplifications....
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