Article
A new approach to HLA-DPB1 typing combining DNA heteroduplex analysis with allele-specific amplification and enzyme restriction.
Immunogenetics - 1 Jan 1992
Sorrentino R, Potolicchio I, Ferrara G B, Tosi R
Abstract excerpt
Allelic polymorphism of HLA-class II antigens plays a key role in the regulation of the immune response and in transplantation immunity. The allelic diversity of these antigens can now be analyzed at the DNA level after amplification by polymerase chain reaction. In this study we apply a simple t...
Topics
- Alleles
- Base Sequence
- Cell Line
- HLA-DP Antigens
- HLA-DP beta-Chains
- Heterozygote
- Histocompatibility Antigens Class II
- Histocompatibility Testing
- Humans
- Molecular Sequence Data
- Nucleic Acid Heteroduplexes
- Oligodeoxyribonucleotides
- Polymerase Chain Reaction
- Polymorphism, Genetic
- Restriction Mapping