Article
Typing of HLA-DQA1 and DQB1 using DNA single-strand conformation polymorphism.
Human immunology - 1 Mar 1992
Carrington M, Miller T, White M, Gerrard B, Stewart C, Dean M, Mann D
Abstract excerpt
The technique of single-strand conformation polymorphism (SSCP), which is capable of distinguishing DNA sequence variability, was adapted to the identification of the HLA-DQA1 and DQB1 alleles. Eight DQA1 alleles and 12 DQB1 alleles were distinguished by amplifying the second exon of the genes in the presence of radioactive deoxynucleotide, denaturing the products with heat, and separating the single strands by...
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