Article
Polymerase chain reaction--single-strand conformation polymorphism analysis of polymorphism in DPA1 and DPB1 genes: a simple, economical, and rapid method for histocompatibility testing.
Human immunology - 1 Feb 1992
Hoshino S, Kimura A, Fukuda Y, Dohi K, Sasazuki T
Abstract excerpt
A new technical trial was carried out to detect polymorphism in HLA-DP genes, based on the diversity in electrophoretic mobility of single-stranded DNA (single-strand conformation polymorphism, SSCP). Genomic DNAs from 31 cell lines homozygous for 2 and 14 different DPA1 and DPB1 alleles, respect...
Topics
- Alleles
- Amino Acid Sequence
- Base Sequence
- Cell Line
- DNA, Single-Stranded
- HLA-DP Antigens
- Histocompatibility Testing
- Humans
- Molecular Sequence Data
- Nucleic Acid Conformation
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length