Allelic typing of the HLA-DR4 group by polymerase chain reaction--single-strand conformation polymorphism analysis.

doi:10.1016/0198-8859(93)90144-p

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Article

Allelic typing of the HLA-DR4 group by polymerase chain reaction--single-strand conformation polymorphism analysis.

Human immunology - 1 Jun 1993

Young N T, Darke C

Abstract excerpt

The technique of SSCP was applied to the molecular subtyping of the HLA-DR4 group of alleles. SSCPs were defined in 16 cell line DNAs, representing the alleles DRB1*0401-*0411 (except DRB1*0409), using group-specific PCR amplification, nondenaturing polyacrylamide gel electrophoresis, and silver...

Topics

Alleles
Base Sequence
DNA, Single-Stranded
Electrophoresis, Polyacrylamide Gel
Genotype
HLA-DR4 Antigen
Humans
Molecular Sequence Data
Oligonucleotide Probes
Polymerase Chain Reaction
Polymorphism, Genetic
Reproducibility of Results
Sensitivity and Specificity

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