Article
Allelic typing of the HLA-DR4 group by polymerase chain reaction--single-strand conformation polymorphism analysis.
Human immunology - 1 Jun 1993
Young N T, Darke C
Abstract excerpt
The technique of SSCP was applied to the molecular subtyping of the HLA-DR4 group of alleles. SSCPs were defined in 16 cell line DNAs, representing the alleles DRB1*0401-*0411 (except DRB1*0409), using group-specific PCR amplification, nondenaturing polyacrylamide gel electrophoresis, and silver staining. Three sets of gel electrophoresis conditions were necessary to differentiate the 10 alleles tested. The SSCPs...
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