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Article

HLA-DQB1 genotyping by a modified PCR-RFLP method combined with group-specific primers.

Tissue antigens - 1 Aug 1991

Nomura N, Ota M, Tsuji K, Inoko H

Abstract excerpt

We previously reported a simple technique for HLA-DQB genotyping by digestion of polymerase chain reaction-amplified genes with restriction endonucleases (PCR-RFLP method). However, this method has some problems in that some heterozygotes cannot be discriminated from each other. Furthermore, concomitantly amplified product derived from the DQB2 gene by the primers used previously also obstructs precise DQB1...

Topics

Alleles
Base Sequence
Blotting, Southern
DNA
Electrophoresis, Polyacrylamide Gel
Endonucleases
Gene Amplification
Genes
Genotype
HLA-DQ Antigens
HLA-DQ beta-Chains
Heterozygote

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Homozygote

Humans

Molecular Sequence Data

Polymerase Chain Reaction

Polymorphism, Restriction Fragment Length

Source

Identifiers

PMID: 1683028
DOI: 10.1111/j.1399-0039.1991.tb01881.x

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1683028-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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