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Article

A simple and rapid method for HLA-DQA1 genotyping by polymerase chain reaction-single strand conformation polymorphism and restriction enzyme cleavage analysis.

Electrophoresis - 1 Nov 1992

Hayashi T, Seyama T, Ito T, Kusunoki Y, Hirai Y, Nakamura N, Akiyama M

Abstract excerpt

A simple and rapid method for identification of alleles at the human leucocyte antigen (HLA)-DQA1 locus is described. The polymorphic second exon of the HLA-DQA1 locus was amplified by the polymerase chain reaction (PCR) method. The amplified DNA was analyzed by single-strand conformation polymorphism (SSCP) and restriction enzyme cleavage assay. Using this method, the eight known DQA1 alleles could be...

Topics

Alleles
Base Sequence
Genotype
HLA-DQ Antigens
HLA-DQ alpha-Chains
Molecular Conformation
Molecular Sequence Data
Polymerase Chain Reaction
Polymorphism, Genetic
Restriction Mapping

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Sequence Alignment

Time Factors

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Identifiers

PMID: 1483431
DOI: 10.1002/elps.11501301191

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1483431-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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