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Article

Massively parallel single-nucleotide mutagenesis using reversibly terminated inosine.

Nature methods - 1 Nov 2016

Haller Gabe, Alvarado David, McCall Kevin, Mitra Robi D, Dobbs Matthew B, Gurnett Christina A

Abstract excerpt

Large-scale mutagenesis of target DNA sequences allows researchers to comprehensively assess the effects of single-nucleotide changes. Here we demonstrate the construction of a systematic allelic series (SAS) using massively parallel single-nucleotide mutagenesis with reversibly terminated deoxyinosine triphosphates (rtITP). We created a mutational library containing every possible single-nucleotide mutation...

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Identifiers

PMID: 27694911
DOI: 10.1038/nmeth.4015
PMCID: PMC5327618

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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