Article
In cell mutational interference mapping experiment (in cell MIME) identifies the 5' polyadenylation signal as a dual regulator of HIV-1 genomic RNA production and packaging.
Nucleic acids research - 18 May 2018
Smyth Redmond P, Smith Maureen R, Jousset Anne-Caroline, Despons Laurence, Laumond Géraldine, Decoville Thomas, Cattenoz Pierre, Moog Christiane, Jossinet Fabrice, Mougel Marylène, Paillart Jean-Christophe, von Kleist Max, Marquet Roland
Abstract excerpt
Non-coding RNA regulatory elements are important for viral replication, making them promising targets for therapeutic intervention. However, regulatory RNA is challenging to detect and characterise using classical structure-function assays. Here, we present in cell Mutational Interference Mapping Experiment (in cell MIME) as a way to define RNA regulatory landscapes at single nucleotide resolution under native...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.