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Article

Radical mutations reveal TATA-box binding protein surfaces required for activated transcription in vivo.

Genes & development - 1 Oct 1996

Bryant G O, Martel L S, Burley S K, Berk A J

Abstract excerpt

Regions on the surface of human TATA-box binding protein (TBP) required for activated transcription in vivo were defined by construction of a library of 89 surface residue mutants with radical substitutions that were assayed for their ability to support activated transcription in vivo, basal transcription in vitro, and TFIIA and TFIIB binding in vitro. Four epitopes were identified in which substitutions in two...

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Source

Identifiers

PMID: 8843200
DOI: 10.1101/gad.10.19.2491

Genes

TBP

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Radical mutations reveal TATA-box binding protein surfaces required for activated transcription in vivo.PMID 8843200Read ↗

A regulated two-step mechanism of TBP binding to DNA: a solvent-exposed surface of TBP inhibits TATA box recognition.Semantic proximity Read ↗

Mutations on the DNA-binding surface of TATA-binding protein can specifically impair the response to acidic activators in vivo.Semantic proximity Read ↗

A common site on TBP for transcription by RNA polymerases II and III.Semantic proximity Read ↗

Core promoter-specific function of a mutant transcription factor TFIID defective in TATA-box binding.Semantic proximity Read ↗

Multiple regions of TBP participate in the response to transcriptional activators in vivo.Semantic proximity Read ↗

Site-specific cross-linking of TBP in vivo and in vitro reveals a direct functional interaction with the SAGA subunit Spt3.Semantic proximity Read ↗

A new regulatory domain on the TATA-binding protein.Semantic proximity Read ↗

A TATA binding protein mutant with increased affinity for DNA directs transcription from a reversed TATA sequence in vivo.Semantic proximity Read ↗

Mutations on the DNA binding surface of TBP discriminate between yeast TATA and TATA-less gene transcription.Semantic proximity Read ↗

A severely defective TATA-binding protein-TFIIB interaction does not preclude transcriptional activation in vivo.Semantic proximity Read ↗

Core promoter-dependent TFIIB conformation and a role for TFIIB conformation in transcription start site selection.Semantic proximity Read ↗

Transcriptional activation by TFIIB mutants that are severely impaired in interaction with promoter DNA and acidic activation domains.Semantic proximity Read ↗

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