Radical mutations reveal TATA-box binding protein surfaces required for activated transcription in vivo.

doi:10.1101/gad.10.19.2491

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Article

Radical mutations reveal TATA-box binding protein surfaces required for activated transcription in vivo.

Genes & development - 1 Oct 1996

Bryant G O, Martel L S, Burley S K, Berk A J

Abstract excerpt

Regions on the surface of human TATA-box binding protein (TBP) required for activated transcription in vivo were defined by construction of a library of 89 surface residue mutants with radical substitutions that were assayed for their ability to support activated transcription in vivo, basal tran...

Topics

Adenovirus E1A Proteins
Animals
Binding Sites
COS Cells
DNA
DNA-Binding Proteins
Epitopes
Fungal Proteins
Genes, fos
Herpes Simplex Virus Protein Vmw65
Humans
Models, Molecular

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mutagenesis, Site-Directed

Mutation

Protein Binding

Receptors, Retinoic Acid

Recombinant Fusion Proteins

Saccharomyces cerevisiae Proteins

Source

Open in PubMed

Identifiers

PMID: 8843200
DOI: 10.1101/gad.10.19.2491

Genes

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-8843200-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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Radical mutations reveal TATA-box binding protein surfaces required for activated transcription in vivo.PMID 8843200Read ↗

A regulated two-step mechanism of TBP binding to DNA: a solvent-exposed surface of TBP inhibits TATA box recognition.Semantic proximity Read ↗

Mutations on the DNA-binding surface of TATA-binding protein can specifically impair the response to acidic activators in vivo.Semantic proximity Read ↗

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Core promoter-specific function of a mutant transcription factor TFIID defective in TATA-box binding.Semantic proximity Read ↗

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Core promoter-dependent TFIIB conformation and a role for TFIIB conformation in transcription start site selection.Semantic proximity Read ↗

Transcriptional activation by TFIIB mutants that are severely impaired in interaction with promoter DNA and acidic activation domains.Semantic proximity Read ↗

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