Article
A TATA binding protein mutant with increased affinity for DNA directs transcription from a reversed TATA sequence in vivo.
Molecular and cellular biology - 1 Dec 2002
Spencer J Vaughn, Arndt Karen M
Abstract excerpt
The TATA-binding protein (TBP) nucleates the assembly and determines the position of the preinitiation complex at RNA polymerase II-transcribed genes. We investigated the importance of two conserved residues on the DNA binding surface of Saccharomyces cerevisiae TBP to DNA binding and sequence discrimination. Because they define a significant break in the twofold symmetry of the TBP-TATA interface, Ala100 and...
Topics
- Adenosine Triphosphatases
- Alanine
- Binding Sites
- Crystallography, X-Ray
- DNA
- DNA Footprinting
- DNA Helicases
- Gene Expression Regulation, Fungal
- Genes, Reporter
- Models, Molecular
- Mutation
- Phenotype
- Proline
- Protein Structure, Tertiary