Article
Gene activation by recruitment of the RNA polymerase II holoenzyme.
Genes & development - 15 Sept 1996
Farrell S, Simkovich N, Wu Y, Barberis A, Ptashne M
Abstract excerpt
The single amino acid "P" (potentiator) mutation in the holoenzyme component GAL11 creates an interaction between that protein and the dimerization region of GAL4. That interaction triggers strong gene activation when the GAL4 fragment is tethered to DNA. Here we show that, among a series of variants of the GAL4 dimerization region and different GAL11P alleles, the strength of the interaction as quantitated in...
Topics
- Bacterial Proteins
- Binding Sites
- DNA-Binding Proteins
- Fungal Proteins
- Gene Expression Regulation
- Mediator Complex
- Models, Genetic
- Mutation
- Peptide Fragments
- RNA Polymerase II
- Recombinant Fusion Proteins
- Saccharomyces cerevisiae Proteins
- Serine Endopeptidases
- Trans-Activators
- Transcription Factors
- Transcriptional Activation