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Article

P22 Arc repressor: enhanced expression of unstable mutants by addition of polar C-terminal sequences.

Protein science : a publication of the Protein Society - 1 Dec 1993

Milla M E, Brown B M, Sauer R T

Abstract excerpt

Many mutant variants of the P22 Arc repressor are subject to intracellular proteolysis in Escherichia coli, which precludes their expression at levels sufficient for purification and subsequent biochemical characterization. Here we examine the effects of several different C-terminal extension sequences on the expression and activity of a set of Arc mutants. We show that two tail sequences, KNQHE (st5) and H6KNQHE...

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Identifiers

PMID: 8298465
DOI: 10.1002/pro.5560021219
PMCID: PMC2142328

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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