Article
P22 Arc repressor: enhanced expression of unstable mutants by addition of polar C-terminal sequences.
Protein science : a publication of the Protein Society - 1 Dec 1993
Milla M E, Brown B M, Sauer R T
Abstract excerpt
Many mutant variants of the P22 Arc repressor are subject to intracellular proteolysis in Escherichia coli, which precludes their expression at levels sufficient for purification and subsequent biochemical characterization. Here we examine the effects of several different C-terminal extension seq...
Topics
- Amino Acid Sequence
- Cell Division
- DNA-Binding Proteins
- Escherichia coli
- Models, Chemical
- Molecular Sequence Data
- Mutation
- Oligopeptides
- Protein Denaturation
- Recombinant Proteins
- Repressor Proteins
- Sequence Homology, Amino Acid
- Suppression, Genetic
- Urea
- Viral Proteins
- Viral Regulatory and Accessory Proteins