Article
GAL4 mutations that separate the transcriptional activation and GAL80-interactive functions of the yeast GAL4 protein.
Genetics - 1 May 1990
Salmeron J M, Leuther K K, Johnston S A
Abstract excerpt
The carboxy-terminal 28 amino acids of the Saccharomyces cerevisiae transcriptional activator protein GAL4 execute two functions--transcriptional activation and interaction with the negative regulatory protein, GAL80. Here we demonstrate that these two functions are separable by single amino acid changes within this region. We determined the sequences of four GAL4C-mutations, and characterized the abilities of...
Topics
- Alleles
- Amino Acid Sequence
- DNA-Binding Proteins
- Fungal Proteins
- Galactose
- Gene Expression Regulation, Fungal
- Genes, Fungal
- Melibiose
- Molecular Sequence Data
- Mutation
- Repressor Proteins