Article
GAL11P: a yeast mutation that potentiates the effect of weak GAL4-derived activators.
Cell - 21 Dec 1990
Himmelfarb H J, Pearlberg J, Last D H, Ptashne M
Abstract excerpt
A mutant yeast in which a weak GAL4-derived activator functions as a strong activator bears a single mis-sense mutation in GAL11 (a.k.a. SPT13). The first 74 amino acids of GAL4, including the zinc-dependent DNA binding region, attached to an acidic activating sequence, are sufficient to respond both to GAL11 and to our mutant GAL11P (potentiator). PPR1, a yeast activator with a similar zinc finger sequence, also...
Topics
- Amino Acid Sequence
- Chromosome Deletion
- DNA, Fungal
- DNA-Binding Proteins
- Fungal Proteins
- Gene Expression Regulation, Fungal
- Mediator Complex
- Models, Genetic
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation