Article
Mutations that increase the activity of a transcriptional activator in yeast and mammalian cells.
Proceedings of the National Academy of Sciences of the United States of America - 1 Mar 1990
Gill G, Sadowski I, Ptashne M
Abstract excerpt
Activating region I of GAL4 protein is a stretch of amino acids, positioned adjacent to the DNA-binding region, that activates transcription in yeast and, as we show here, in mammalian cells. Here we describe mutations located throughout a 65-amino acid region that increase the activation function of region I. Most of these mutations replace positively charged amino acids in the region with neutral ones, although...
Topics
- Amino Acids
- Animals
- Cell Line
- Chromosome Deletion
- Cloning, Molecular
- DNA-Binding Proteins
- Fungal Proteins
- Mutation
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins
- Transcription Factors