Article
Antibiotic resistance mechanisms of mutant EF-Tu species in Escherichia coli.
Biochemistry and cell biology = Biochimie et biologie cellulaire - 1 Jan 2000
Kraal B, Zeef L A, Mesters J R, Boon K, Vorstenbosch E L, Bosch L, Anborgh P H, Parmeggiani A, Hilgenfeld R
Abstract excerpt
Analysis of antibiotic-resistant EF-Tu mutants has revealed a connection between resistance and structural elements that participate in the GTPase switching mechanism. Both random and site-directed mutagenesis methods have yielded sets of purified mutant EF-Tu resistant to kirromycin (kirT) or pulvomycin (pulT). All kirT mutations cluster in the interface of domain 1 and 3 of EF-Tu in its GTP-bound conformation,...
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